Microbiology Laboratory

Microbiology Laboratory which has a fundamental role in the diagnosis, treatment, follow-up and control processes of the diseases is equipped with the technical equipment, where 350 separate tests from a wide spectrum can be applied, at international standards by closely following the changes and innovations in its field in order to maximize the quality of education.

Microbiology Laboratory consist of 7 different laboratories.

In Microscopy Laboratory, the procedures such as staining, microscopic examination and identification of samples collected from various cultures are conducted.

In Sterilization Laboratory, the demonstration of how to sterilize the contaminated materials and sterilization steps are taught.

In Microorganism Identification Laboratory; various identification procedures concerning the microorganisms made from cultured samples.

In Immunofluorescence Microscope Laboratory; the stimulation of samples by utilizing fluorescent markers and examination/marking of cell parts and microorganisms.

In Culturing Laboratory, procedures concerning which process will be used for the samples and which media they will be cultured is taught, and incubation in appropriate temperatures.

In Culture Media Preparation Laboratory, the culturing media which will establish a appropriate environment for microorganisms to reproduce.

Administrative Staff

Vice Rector

Prof. Dr. Ahmet İLVAN

[email protected]

444 1 428 - 56102

Dean

Prof. Dr. Özgün ENVER

[email protected]

444 1 428 - 28701

Laboratories Coordinator

Öğr. Gör. Veli Bülent UÇAR

[email protected]

444 1 428 - 14628

Laboratuvar Koordinatör Yardımcısı

Melike ÖZDEMİR CENGİZ

[email protected]

444 1 428 - 56015

Medical Laboratory Technician

Gaye ÇEKİÇ

[email protected]

444 1 428 - 56010

Medical Laboratory Technician

Vedat ÇALIŞKAN

[email protected]

444 1 428 - 57005

Academic Staff

Department Head

Prof. Dr. Yaşar Ali ÖNER

[email protected]

444 1 428 - 52602

Dr.

Esat BONABİ

[email protected]

444 1 428 - 57101

Dr.

Özer AKGÜL

ozerakgü[email protected]

444 1 428 - 57106

Dr.

Burcu SAPMAZ

[email protected]

444 1 428 - -

Dr.

Silva POLAT SARI

[email protected]

444 1 428 - 28502

Lecturer

Özge ALTINOK

[email protected]

444 1 428 - 52752

Culture Media Preparation Laboratory

Air sampler

General Information It is used for determining the microbial load in the air. The equipment operates in accordance with the “Anderson’s Collision Principle”.
Brand & Model Merck - MAS-100 ECO
Technical Details

Air current 100 L/min (Aspiration volume)
Petri Dish Standard 100 mm Petri Plate
Operation time 3 hour max.
16 character indicator LCD screen

Colony counter

General Information It is used for quickly and easily counting the microorganisms produced on the petri plate. Monitored surface could be observed in detail by the magnifier.
Brand & Model Stuart – SC6T
Technical Details

230 V 50 Hz

Magnifier

Magnetic Mixer

General Information It is used for the dissolution and mixing of the materials in a prepared solution by adjusting the temperature and cycle with the use of magnetic fish.
Brand & Model Dragon – MS-H-S
Technical Details

Capacity 20 L
Cycle 0-1500 rpm
Temperature Max 340 °C
Heating time for 1 liter of water 6 K/min
Analog Cycle and Temperature Setting
350°C Mechanical Heat Stopper for Safety

Orbital Shaker

General Information It is used for homogeneously and cyclical shaking of the liquids in glass containers in various shapes and volumes.
Brand & Model Stuart -SSL1
Technical Details

Speed range 30 to 300 rpm
Operational temperature range +4°C to +40°C
Maximum load 10 kg
Maximum humidity allowed %80 230V, 50 Hz

230V, 50 Hz

DNA/RNA Isolation Laboratory

Shaker Double Boiler

General Information It is used for slowly heating the samples in a fixed temperature, incubation or preserving its temperature without directly subjecting the sample to the fire during research and practices.
Brand & Model Nükleon – NCS 30
Technical Details

Tank Capacity 30 L
Operation temperature range +5 ° C/ + 99.9 ° C
Speed cycle 20-250 rpm
Horizontal shaking motion
Timer 1 min – 99 hours 59 min + Continuous operation
Stainless steel interior
230 V, 50Hz

Mini Centrifuge

General Information It is used for collecting cells, blood works and DNA collection. Heating of the samples after the operation is prevented by the automatic door, and easy access to the samples is established.
Brand & Model Eppendorf AG Minispin
Technical Details

Speed 800-13.400 rpm (100 rpm steps)
Rotor capacity 12 x 1.5 /2 ml container, 2 x PCR string
Short cycle button for quick and easy centrifuge

Speeding time 13 s
Stopping time 12 s
15 secs – 30 min timer

230V, 50/60 H<, 70 W

Cooler centrifuge

General Information It is an electrical motor equipment used for fixed axis cyclical turning motion. High cycle of the equipment helps with the separation of the solutions with the effects of centrifugal force.
Brand & Model Thermo Scientific Megafuge 16R
Technical Details

Temperature range -10 °C  +40 °C,
Operation time 9 hours 99 minutes
Speed range 9
Stopping range 10
CSA Certified, CE Documented

230 V, 50/60 Hz

Ultrasound Bath

General Information The formation of gas bubble by the ultrasound soundwaves is called “cavitation”, and the pop of these bubbles is called “acoustic cavitation”. The shockwaves caused by this popping creates a brushing effect on the surfaces. Thus, helps with the quick and precise cleaning by reaching deep corners, cracks and areas which are not possible to reach with other methods.
Brand & Model Protechology
Technical Details

4 L Capacity
300 W Heating power
20-90 °C Temperature range, 0-60 minute timer
220 V/50 Hz Power source

Vortex

General Information It is used for mixing the reagents of a test and a sample or thinner, and to provide homogenous mixture.
Brand & Model Scilogex MX-S
Technical Details

250-2500 rpm

Allowed environment temperature 5-40 ° C
Shaded pole motor

110 V, 60 Hz

Immunofluorescence Microscope Laboratory

Immunofluorescence Microscope

General Information It is used for stimulating the samples by fluorescent markers and process through filters, and examination/marking of cell parts and microorganisms, and for obtaining information concerning the information about molecule distribution and quantity
Brand & Model ZEISS - Scope.A1 AX10
Technical Details

Operation environment temperature -10 °C to +40 °C
Operation relative humidity range 35 °C/max. 75 %
3 different filter (DAPI, FITC, and RHODAMINE).
5 different objective (5X, 10X, 20X, 40X and100X).
Obtained images are saved as a photo with the computer software

Stereo Microscope

General Information It is a microscope operating as a fixed binocular rather than light microscope and used for examining the samples without glass slide and cover glass and used for collecting 3D imagery. It is possible to obtain high contrasted, bright and clear images as well as wide objective in industrial and clinical studies with its ergonomic design.
Brand & Model MOTIC - SFC-11A N2GG
Technical Details

45º angle, 360º rotatable binocular headpiece
Wide angle eyepiece WF10X / 20mm
Magnifying 10X-20X
Operation distance 95 mm
Pole and headpiece holder base stand
Rough focus with tension setting
Main feed 220V-240V (CE)
White and frosted glass sheets

Culturing Laboratory

Drying Oven (Incubator)

General Information It is an equipment used for reproducing microorganisms, sterilization process, heating, drying, and making chemical reactions between 5°C and 75°C.
Brand & Model Thermo Scientific - Heraterm IGS100
Technical Details

Temperature range: 5°C to 75°C
Loading capacity per shelf: 25/55 kg/Ibs
Natural noise none
Heating time: 25 minutes
Max. 16 shelf
120V / 4.5A / 540W

McFarland Densitometer

General Information It is used for determining the microorganism sensitivity to antibiotics, microorganism identification with various tests, measuring the turbidity of cell suspension and quantitative estimation.
Brand & Model BIOSAN – Den-1
Technical Details

Measuring range: 0.3-15.0 McF
Display resolution: 0.1 McF
LED light source
Measuring wavelength: λ = 565 ± 15 nm
Accuracy: ±% 3
Measuring time: 1 s
Example volume: Not less than 2 ml
Tube outer diameter: 16 mm-18 mm

12V, 80 mA/1 W

Cooling Drying Oven

General Information It is used for incubation, biotechnology research and quality control by homogenously distributing the adjusted lower temperature.
Brand & Model Thermo Scientific – BK 6160
Technical Details

Capacity: 166L
Temperature range: 0°C to 50°C
Shelf: 2
Cooler: R134a; 220g capacity
230V / 50 Hz
Relative humidity: 5 °C’ de <75; 37 °C’ de <90

Microbiology/Medical Biology and Genetics Laboratory

-90°C Deep Freeze

General Information It is used for storing the samples by preserving their features for a long period of time in ultra-lower temperatures. The cooling process can be conducted in precise temperatures between -45°C to -90°C.
Brand & Model Nüve – DF 590
Technical Details

Capacity 560 L
Temperature adjustment range -45°C/-90°C
Temperature alarm range +5/25°C
Auditory and visual power failure alarm
Auditory and visual open-door alarm
Automatic charged 12 hour battery

Stainless steel interior

4 wheels and 2 anchor wheel

Microorganism Identification Laboratory

Biological Safety Cabin

General Information It is used for procedures such as preventing the distribution of harmful biological matters, protection of users and samples, microbiology, genetic combination and analyses of samples with biological danger which requires a sterile environment.
Brand & Model Thermo Scientific – Safe 2020
Technical Details

H14 HEPA EN 1822, most penetrating particle size (MPPS) 99,995%
Max. 40 °C room temperature
Air flow speed: Input 0.45 m/sec, Output 0.36 m/sec

230 V - 50/60 H

Identification device

General Information VITEK® MS uses innovative mass spectrometry technology in automatic microbial identification (Matrix Assisted Laser Desorption Ionization Time-of-Flight-MALDI-TOF). Preparation station is used in the preparation of the samples. This station contains the records of prepared materials. Status of the device could be observed in the collection station and sample spectrums are obtained. These are sent to the MYLA for analysis. The microorganisms are expected to be identified within minutes.
Brand & Model Biomerieux – VITEK MS
Technical Details

337 nm nitrogen laser
Max. pulse rate – 50 Hz (50 laser shots per second)
Temperature 18° - 26° C
Relative humidity %70 non-condensing
4 slide capacity for 1 turn and 192 isolate test (48 sample spot x 4 slides)
230 VAC, 50/60 Hz, 1000 VA single phase
Weight 330 Kg

Microscopy Laboratory

Light microscope with visual attachment

General Information It is used for reflecting the visuals to LCD screens in order for students to see. It is used in photographing, editing and recording the images through computer software
Brand & Model Olympus CX41 RF
Technical Details

30-60° Tube angle
48-75 mm pupillary distance
100-120 / 220-240 V,
0.42 / 0.25 A, 50/60 Hz

Light microscope

General Information It is used for magnifying the images of objects and increasing the clarity by adjustment and utilizing the lenses, and used for biological research.
Brand & Model Olympus CX22 RFS1
Technical Details

30° Tube angle
48-75 mm pupillary distance
Upper limit stopper feature
100 - 120 / 220 - 240 V, 0.42 / 0.25 A, 50/60 Hz

PCR Room

ELİSA Well Wash

General Information It is used for washing the cell cultures, protein lines, Western blots and beads as well as DNA purification protocols. The equipment determines the microplate format compatibility, number of canal heads and distributed liquids, and aspirated volume range; helps with programming the cycles and durations, well selection for the customized procedures.
Brand & Model Thermo Scientific Well Wash
Technical Details

50-1000 uL Wash capacity

1x8 washing heads
5 to 100 ml Prime capacity
<%5 accuracy
USB connection
LCD display

50/60 Hz, 100-240 V
CE documented

Spectrophotometer/ELISA Reader

General Information It is used for measuring the quantity of the substance which is looked for in the solution by identifying the intensity of light penetrated through the solution. The analysis is conducted by correctly placing the sample and adjusting the desired wavelength.
Brand & Model Thermo Scientific Multiskan Go
Technical Details

200-1000 nm
6 seconds with 96 well plate, 10 second measuring with 384 well plate
Max: <110w power consumption, <2.5w power save
Reading range up to 4 Abs
Wavelength resolution 1 nm
Wavelength accuracy 2 nm
ıncubation temperature range 2 ° to 45 ° C
Both microscope and cuvette range
Multiple language selection

Sterilization Laboratory

Steam sterilization

General Information The equipment performs the sterilization procedure by temperature higher than 100°C in a high pressure and temperature environment saturated by steam. Condensation of the steam instead of evacuation during the sterilization procedure is established by the cooling system, and the prevented sudden drops in pressure helps with avoiding the boiling-overflowing of the liquids.
Brand & Model Nüve OT 40L
Technical Details

Cell capacity 40 L
Sterilization range 105 °C – 135 °C
Pre-heating temperature 40°C-60 °C
Sterilization period 1-300 minutes
5 preset sterilization program
Delayed start
Date and time adjustment
230 V / 50 Hz

Washing machine

General Information It is used for hand-free washing of the glass containers, measuring cups, pots and gadgets (spatula, etc.).
Brand & Model Miele – G7883
Technical Details

Resistance drying system
10 programs
Micro sensor remote
400 liter/minute circulation pump
Hot and cold water installment max. 70°C

Oven

General Information It is used for the sterilization through boiling of the materials used in laboratory and cleaned after the use.
Brand & Model Profilo - FRS 3010GTL Turbo
Technical Details

4 gas unit (2 normal, 1 strong, 1 economic)
Button automated firing

Gas safety
Steel grate bar
Glass top lid
3D turbo oven
2 cooking program (Hot air, defrost)
A energy

Dry Air Sterilization

General Information It is used for sterilizing all of the devices which can be sterilized through hot air.
Brand & Model Thermo Scientific - Heratherm OGS180
Technical Details

Temperature range 50 ° C to 250 ° C
Internal timer
Capacity 176 L
Temperature change: ±4.0 (at 150 °C)
Total load per shelf 25 kg
120 V / 60 Hz / 14 A

UV Pure Water

General Information It is a device used for producing high quality water rather than tap water in procedures such as chromatography, elemental analysis, tissue culture, molecular biology and biochemistry, ant autoclave.
Brand & Model Merck Millipore - Direct Q5UV
Technical Details

Production flow 5 L/hour 25°C +/- %15
Flow speed >0.5 L /minute
Resistance measurement 18,2 ΜΩ cm in 25 ° C
Tap Water Pressure from 0.5 to 6 bar
Tip I or Tip III
UV measurement
100-250 V

Rezervasyon Takvimi
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Makale Başlığı

02.02.2020 Detaylar

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LaboratuvarCihaz AdıTest Adı/Tedavi Parametreleri/Yapılan Uygulamalar
DNA/RNA Isolation LaboratorySpectrophotometer/ELISA ReaderELİSA
Immunofluorescence Microscope LaboratoryImmunofluorescence MicroscopeDark field examination
Culture Media Preparation LaboratoryDrying Oven (Incubator)Throat Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Urine Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Stool Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Ear Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Wound Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Phlegm Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Nose Culture
Culture Media Preparation LaboratoryDrying Oven (Incubator)Other Cultures (Fungus, …)
Culture Media Preparation LaboratoryDrying Oven (Incubator)Blood Culture (Aerobe, Anaerobe)
Culture Media Preparation LaboratoryDrying Oven (Incubator),Light microscope, Light microscope with visual attachmentBacteria Identification (Manual)
Microscopy LaboratoryLight microscope, Light microscope with visual attachmentStained Microscopic Exam (Gram, M. Blue, Wright, Ziehl-Neelsen, etc.)
Microscopy LaboratoryLight microscope, Light microscope with visual attachmentNon-stained Direct Microscopic Examination
Microscopy LaboratoryLight microscope, Light microscope with visual attachmentDirect Parasite Examination (Manual)
Microscopy LaboratoryLight microscope, Light microscope with visual attachmentFungus Exam (KOH)
Microscopy LaboratoryLight microscope, Light microscope with visual attachmentMicro bacteria Exam (ARB) Direct Microscopy
Microscopy LaboratoryDrying Oven (Incubator)Antibiotic Sensitivity Test
Microscopy Laboratory_Catalase Exam
Microorganism Identification LaboratoryDrying Oven (Incubator)Manual Identification Test (API, Oxidase, …)
Istanbul Aydin University Halit Aydın Kampüsü No:38 , Sefaköy–Küçükçekmece, Istanbul, 34295 Tel: 444 1 428

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