Medical Biology and Genetic Laboratory is equipped to perform the various genetic procedures such as examination of organisms on molecular level, DNA and RNA isolation/duplication, spectrophotometric and electrophoresis techniques in order to clarify the reasons behind diseases, to obtain information concerning diagnosis and treatment as well as research.
Medical Biology and Genetic Laboratory consist of 4 separate laboratories.
In Microscopy Laboratory, prepared samples are examined under the microscope. In DNA/RNA Isolation Laboratory, DNA/RNA are isolated manually or by kits.
In PCR Laboratory, certain parts of isolated DNA/RNA are duplicated in special tubes. Spectrophotometric measurements are also conducted.
In Electrophoresis Laboratory, migration of loaded molecules in an electrical field or gel electrophoresis result imaging of PCR samples are conducted.
In Cell Culture Laboratory, molecular reasons of the diseases as well as biological activities of candidate biomolecules in in vitro cultured cells are researched.
| General Information | It is used for the dissolution and mixing of the materials in a prepared solution by adjusting the temperature and cycle with the use of magnetic fish. |
| Brand & Model | Dragon – MS-H-S |
| Technical Details | Capacity 20 L |
| General Information | It is used for homogeneously and cyclical shaking of the liquids in glass containers in various shapes and volumes. |
| Brand & Model | Stuart -SSL1 |
| Technical Details | Speed range 30 to 300 rpm 230V, 50 Hz |
| General Information | It is used for slowly heating the samples in a fixed temperature, incubation or preserving its temperature without directly subjecting the sample to the fire during research and practices. |
| Brand & Model | Nükleon – NCS 30 |
| Technical Details | Tank Capacity 30 L |
| General Information | It is a digital temperature and timer controlled equipment used for DNA and enzyme analyses, agar incubation and cell culture analyses. |
| Brand & Model | Allsheng 1685E- Dijital Soğutuculu |
| Technical Details | 3 mm Orbital shaking motion 25 rpm mixing accuracy 150W/240V |
| General Information | It is used for collecting cells, blood works and DNA collection. Heating of the samples after the operation is prevented by the automatic door, and easy access to the samples is established. |
| Brand & Model | Eppendorf AG Minispin |
| Technical Details | Speed 800-13.400 rpm (100 rpm steps) Speeding time 13 s 230V, 50/60 Hz, 70 W
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| General Information | It is an electrical motor equipment used for fixed axis cyclical turning motion. High cycle of the equipment helps with the separation of the solutions with the effects of centrifugal force. |
| Brand & Model | Thermo Scientific Megafuge 16R |
| Technical Details | Temperature range -10 °C +40 °C, 230 V , 50/60 Hz |
| General Information | It is used for the temperature control of fast heating and cooling steps in various tests. |
| Brand & Model | Eppendorf - Thermostat Plus |
| Technical Details | Temperature range -5° C to 99 ° C 100-240 V, 0.8 A, 50/60 Hz, 75W |
| General Information | The formation of gas bubble by the ultrasound soundwaves is called “cavitation”, and the pop of these bubbles is called “acoustic cavitation”. The shockwaves caused by this popping creates a brushing effect on the surfaces. Thus, helps with the quick and precise cleaning by reaching deep corners, cracks and areas which are not possible to reach with other methods. |
| Brand & Model | Protechology |
| Technical Details | 4 L Capacity |
| General Information | It is used for mixing the reagents of a test and a sample or thinner, and to provide homogenous mixture. |
| Brand & Model | Scilogex MX-S |
| Technical Details | 250-2500 rpm Allowed environment temperature 5-40 ° C 110 V, 60 Hz |
| General Information | Vertical Electrophoresis Electrophoretic analysis is a procedure based on the migration principle of molecules that are dissolved in an electrical field by their electrical load. The equipment is compatible with small, medium and large molecules for electrophoretic separation of polyacrylamide gels prepared with acrylamide polymerization. |
| Brand & Model | Thermo Scientific - 7339 P8DS-2 |
| Technical Details | Gel dimensions: 10 x 8-10 cm |
| General Information | It is an equipment used for reproducing microorganisms, sterilization process, heating, drying, and making chemical reactions between 5°C and 75°C. |
| Brand & Model | Thermo Scientific - Heraterm IGS100 |
| Technical Details | Temperature range: 5°C to 75°C |
| General Information | It is used for imaging the fragments under electrophoresis under UV light. |
| Brand & Model | Vilber - E box VX5 Serial N |
| Technical Details | Digital Camera Automated UV stopping system when the lid is open, safety latch UV stopping after 20 minutes of operation |
| General Information | It is used for boiling the gel for gene studies. |
| Brand & Model | Arçelik - MD-574 |
| Technical Details | Oven capacity: 17 l |
| General Information | Electrophoretic analysis is a procedure based on the migration principle of molecules that are dissolved in an electrical field by their electrical load. Horizontal electrophoresis tank helps with separating the molecules by their size, structure, environmental density, ionic force and applied current. |
| Brand & Model | Thermo Scientific - 7309 B1 |
| Technical Details | CE Documented |
| General Information | |
| Brand & Model | |
| Technical Details |
| General Information | It is used for stimulating the samples by fluorescent markers and process through filters, and examination/marking of cell parts and microorganisms, and for obtaining information concerning the information about molecule distribution and quantity. |
| Brand & Model | ZEISS - Scope.A1 AX10 |
| Technical Details | Operation environment temperature -10 °C to +40 °C |
| General Information | It is a microscope operating as a fixed binocular rather than light microscope and used for examining the samples without glass slide and cover glass and used for collecting 3D imagery. It is possible to obtain high contrasted, bright and clear images as well as wide objective in industrial and clinical studies with its ergonomic design. |
| Brand & Model | MOTIC - SFC-11A N2GG |
| Technical Details | 45º angle, 360º rotatable binocular headpiece |
| General Information | It is used for storing the samples by preserving their features for a long period of time in ultra-lower temperatures. The cooling process can be conducted in precise temperatures between -45°C to -90°C. |
| Brand & Model | Nüve – DF 590 |
| Technical Details | Capacity 560 L Stainless steel interior 4 wheels and 2 anchor wheel |
| General Information | It is used for procedures such as preventing the distribution of harmful biological matters, protection of users and samples, microbiology, genetic combination and analyses of samples with biological danger which requires a sterile environment. |
| Brand & Model | Thermo Scientific – Safe 2020 |
| Technical Details | H14 HEPA EN 1822, most penetrating particle size (MPPS) 99,995% 230 V - 50/60 H |
| General Information | It is used for reflecting the visuals to LCD screens in order for students to see. It is used in photographing, editing and recording the images through computer software. |
| Brand & Model | Olympus CX41 RF |
| Technical Details | 30-60° Tube angle |
| General Information | It is used for magnifying the images of objects and increasing the clarity by adjustment and utilizing the lenses, and used for biological research. |
| Brand & Model | Olympus CX22 RFS1 |
| Technical Details | 30° Tube angle |
| General Information | It is used for washing the cell cultures, protein lines, Western blots and beads as well as DNA purification protocols. The equipment determines the microplate format compatibility, number of canal heads and distributed liquids, and aspirated volume range; helps with programming the cycles and durations, well selection for the customized procedures. |
| Brand & Model | Thermo Scientific Well Wash |
| Technical Details | 50-1000 uL Wash capacity 1x8 washing heads |
| General Information | It is used for measuring the quantity of the substance which is looked for in the solution by identifying the intensity of light penetrated through the solution. The analysis is conducted by correctly placing the sample and adjusting the desired wavelength. |
| Brand & Model | Thermo Scientific Multiskan Go |
| Technical Details | 200-1000 nm 6 seconds with 96 well plate, 10 second measuring with 384 well plate |
| General Information | It is used for amplifying the DNA and RNA particles by polymerase chain reaction (PCR). It is used for quickly and precisely increasing and decreasing the temperature of the samples contained in a block, results in denaturation and reconnection with various reagents. Amplified genetic material can be used in procedures such as cloning, sequencing, expression analysis and genotyping. |
| Brand & Model | Techne – 5PrimeG / 02 |
| Technical Details | 96 well |
| General Information | It is a device used for producing high quality water rather than tap water in procedures such as chromatography, elemental analysis, tissue culture, molecular biology and biochemistry, ant autoclave. |
| Brand & Model | Merck Millipore - Direct Q5UV |
| Technical Details | Production flow 5 L/hour 25°C +/- %15 |
| Laboratuvar | Cihaz Adı | Test Adı/Tedavi Parametreleri/Yapılan Uygulamalar |
|---|---|---|
| DNA/RNA Isolation Laboratory | Mini Centrifuge, Vortex, Shaker Double Boiler | Genomic DNA Isolation |
| PCR Room | Spectrophotometer | DNA Concentration Calculation |
| PCR Room | PCR | Oligonucleotide Synthesis |
| PCR Room | PCR | Gene Amplification |
| Electrophoresis Laboratory | Vertical Electrophoresis | Agarose Gel Electrophoresis |
| Electrophoresis Laboratory | Gel Imaging System | Agarose Gel Electrophoresis Imaging |
| PCR Room | PCR | Mutation Analysis with Restriction Enzymes |
| PCR Room | PCR | DNA Sequence Analysis, Sequencing |
| PCR Room | PCR | All Genome, Single and Multiple Gene Expression Analysis |
| PCR Room | _ | Bioinformatics Analysis |
| DNA/RNA Isolation Laboratory | Mini Centrifuge, Vortex, Shaker Double Boiler | DNA Isolation from Plant |
